iolQ [2019-09-06 09:43:11]

transcriptional repressor of [gene|AA7DCEFB17FD6F9C4F65235E9A247A6EC2242B06|iolX] expression, transcriptional activator involved in the degradation of glutamine phosphoribosylpyrophosphate amidotransferase

locus: BSU_10840

pI: 5.78

mw: 36.44 kDa

protein length: 337 aa

gene length: 1014 bp

function: control of scyllo-inositol utilization

product: transcription factor ([SW|LacI family])

essential: no

synonyms: degA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.16|Utilization of inositol]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 1,163,148 1,164,161

The protein

Catalyzed reaction/ biological activity

• binds to [gene|AA7DCEFB17FD6F9C4F65235E9A247A6EC2242B06|iolX] promoter region [pubmed|28693424]

Structure

• [PDB|2HSG] ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA], 35% identity) [pubmed|17500051]

Expression and Regulation

Operons

• genes: [gene|5B173B400D0B4C150E94A98D35E27B0B4358489B|yisR]-[gene|17F5188958A415D8D09439E02E1D1E0661B5C760|iolQ]
  description: [pubmed|22383849]

additional information

• the mRNA is very stable (> 15 min) [pubmed|12884008]

Biological materials

Mutant

• BKE10840 ([gene|17F5188958A415D8D09439E02E1D1E0661B5C760|iolQ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATGTTCATCCCTCGTC, downstream forward: _UP4_TGAGCCCGCTAATGAGCGGG
• BKK10840 ([gene|17F5188958A415D8D09439E02E1D1E0661B5C760|iolQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATGTTCATCCCTCGTC, downstream forward: _UP4_TGAGCCCGCTAATGAGCGGG

References

• 8407808,12884008,28693424